| 淸肝解酒湯이 CYP2E1-transfected HepG2 cell에서 알코올유발 세포독성에 미치는 영향 |
| 이지은, 이장훈, 김영철, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| The Effects of Chungganhaeju-tang(Qingganjiejiu-tang) on Alcohol induced Cytotoxicity in CYP2E1-transfected HepG2 cells. |
| Ji-eun Lee, Jang-hoon Lee, Young-chul Kim, Hong-jung Woo |
| Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University |
| Correspondence |
Jang-hoon Lee ,Tel: 02-958-9118, Fax: 02-958-9120, Email: hjwoo@khu.ac.kr
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Received: February 10, 2006, Accepted: February 23, 2006, Published online: March 30, 2006. |
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| ABSTRACT |
Objectives:
Induction of CYP2E1 by ethanol is believed to be one of the major mechanism by which ethanol generate a state of oxidative stress. Previous studies showed that treatment with Chungganhaeju-tang prevents hepatic inflammation and apoptosis in alcoholic liver disease. The purpose of our study is to determine if Chungganhaeju-tang can also protect against alcohol-induced cytotoxicity in CYP2E1-transfected HepG2 cells.
Materials and Methods :
CYP2E1-transfected HepG2 cells and control vector-transfected HepG2 cells were exposed for isx hours to Chungganhaeju-tang, and then 50 mM of ethanol was added and left for two days.
Results:
Ethanol significantly decreased cell viability in CYP2E1-transfected HepG2 cells and increased apoptosis. These alterations were attenuated by Chungganhaeju-tang. This was accompanied by an improvement of NF-κB and Akt activation.
Conclusion:
These results suggest that Chungganhaeju-tang exerts inhibitory effect against the cytotoxicity induced by alcohol in CYP2E1-transfected HepG2 cells, and that this is a protective action due, at least in part, to an activation of NF-κB that plays a key role in the protection mechanism, and in reducing hepatotoxic cytokine gene expression. |
| Key words:
Chungganhaeju-tang(Qingganjiejiu-tang), CYP2E1, ethanol, apoptosis, Akt, NF-κB |
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