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The Journal of Internal Korean Medicine 2008;29(2): 469-489. |
| 淸肝解酒湯이 알코올 유발 간섬유화와 단백질 발현에 미치는 영향 |
| 전재현, 김영철, 이장훈, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| The Effects of Chungganhaeju-tang(Qingganjiejiu-tang) on Alcoholic Liver Damages by Applying Proteomics |
| Jae-hyun Jun, Young-chul Kim, Jang-hoon Lee, Hong-jung Woo |
| Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University |
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Published online: June 30, 2008. |
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| ABSTRACT |
Objectives:
The purpose of this study was to investigate the effects of Chungganhaeju-tang(Qingganjiejiu-tang) on alcoholic liver damaged by applying proteomics.
Materials and Methods :
Sprague-Dawley rats were used in this experiment the rats were divided into the normal group, the control group(alcohol) and the sample group(CGHJT +alcohol). The ethanol was orally administered twice a day for 6 weeks in the control and sample groups. Water instead of ethanol was orally administered twice a day for 6 weeks in the normal group. CGHJT extract was orally administered once a day for 6 weeks in the sample group. The livers of each group were processed and assessed by histology, Western Blot, OxyblotTM, CBB and 2-dimensional electrophoresis.
Results:
In the histological findings of the liver, CGHJT inhibited hepatic fibrogenesis induced by alcohol. TIMP-1 decreased in the sample group assessed by western blot and statistical significance was noted by dot blotting(p<0.05). In the OxyblotTM, protein oxidation induced by alcohol treatment decreased with CGHJT. In the 2-dimensional electrophoresis finding, increased proteins alcohol such as HSP 60, 60kDa heat shock protein, 3-mercaptopyruvate sulfurtransferase were normalized by CGHJT. CGHJT was considered to normalize the anti-oxidation activity elevated by alcohol. In the 2-dimensional electrophoresis finding, increased oxidized proteins such as actin, prolyl 4-hydroxylase beta polypeptide, 94kDa glucose regulated protein(GRP94), heat shock protein 90-alpha(HSC86), calreticulin precursor(CRP55), ATP synthase beta chain mitochondrial precursor, caspase-8 precursor, and dihydrolipoamide succinyltransferase(E2) decreased with CGHJT. CGHJT was considered to reduce the oxidative stress of alcohol.
Conclusion:
Chungganhaeju-tang(Qingganjiejiu-tang) exerts an inhibitory effect against the fibrosis and protein oxidation induced by alcohol treatment of rat liver. CGHJT was considered to normalize the elevated anti-oxidation activity by alcohol and to reduce the level of oxidative stress due to alcohol. |
| Key words:
Chungganhaeju-tang(Qingganjiejiu-tang), proteomics, alcohol, liver fibrosis |
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