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The Journal of Internal Korean Medicine 2008;29(4): 835-845. |
| 五味子(Fructus Schisandrae)가 흰쥐 해마신경세포의 저산소증 모델에서 세포사에 미치는 영향 |
| 주대환1, 신길조1, 문일수2 |
1동국대학교 한의과대학 내과학교실
2동국대학교 의과대학 해부학교실 |
| Prevention of ROS Production and MMP Dissipation by Fructus Schisandrae(FS) in a
Hypoxic Model of Cultured Rat Hippocampal Neurons |
| Dae-hwan Ju1, Gil-cho Shin1, Il-soo Moon2 |
1Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University
2Dept. of Anatomy, College of Medicine, Dongguk University |
| Correspondence |
Gil-cho Shin ,Tel: 031-710-3710, Fax: 031-710-3780, Email: shingcho@naver.com
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Published online: December 30, 2008. |
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| ABSTRACT |
Objectives:
The purpose of this study was to investigate the effect of FS for the modulation of ROS and MMP in a hypoxic model of cultured rat cortical cells.
Methods :
For the effect of FS on the viability, FS was added to culture media (neurobasal supplemented with B27) and cell viability was measured by LDH assay. To investigate the effects of FS on ROS generation and MMP preservation, cells grown in FS-containing media were given a hypoxic shock(2% O2/5% CO2, 37℃, 3 hrs) on DIV 10, stained with H2DCF-DA(10 nM) and JC-1, respectively, and observed by fluorescent microscope.
Results:
1. FS has a protective effect of cortical cells in both normoxia and hypoxia. 2. FS reduced the generation of ROS and this reduction was especially significant at 3 days after hypoxia. 3. FS was effective for the maintenance of MMP in hypoxia, and this efficacy was especially significant at 3 days after hypoxia.
Conclusions:
Taken together, these results indicate that FS attenuates ROS generation and MMP dissipation, which eventually protects from neuronal cell death in hypoxia. |
| Key words:
Fructus Schisandrae, ROS, MMP |
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