| 茵蔯清肝汤이 간성상세포의 섬유화 억제에 미치는 영향 |
| 이홍일, 김영철, 우홍정, 이장훈 |
| 경희대학교 한의과대학 간계내과학교실 |
| Inhibitory Effect of Injinchunggan-tang(Yinchenqinggan-tang) on Fibrogenesis of Rat Hepatic Stellate Cells |
| Hong-ri Lee, Young-chul Kim, Hong-jung Woo, Jang-hoon Lee |
| Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University |
| Correspondence |
Young-chul Kim ,Tel: 02-958-9118, Fax: 02-958-9120, Email: yckim@khmc.or.kr
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Published online: March 30, 2009. |
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| ABSTRACT |
Objectives:
This study was performed to investigate the anti-fibrogenic effect of Injinchunggan-tang on cultured rat hepatic stellate cells.
Materials and Methods :
Hepatic stellate cells(HSC-T6) were treated with various concentrations of Injinchunggan-tang extract for 24, 48, and 72 hours. The extraction was done with distilled water. After the treatment, cell viability, proliferation, procollagen levels and the mRNA of the TIMP-1, TIMP-2, and ASMA were measured by using MTT assay, BrdU assay, procollagen type I C-peptide EIA kit and RT-PCR.
Results:
The proliferation, mRNA expression and synthesis of collagen of the hepatic stellate cells were inhibited by Injinchunggan-tang treatment in a dose-dependent manner. This indicates the prescription has inhibitory effect on fibrogenesis of the liver by regulating the fibrogenesis associated genes in transcription. Cell viability was inhibited in time- and dose-dependent manners. It seemed that the drug should be used with sufficient dose to acquire treatment effect.
Conclusion:
These results suggest that Injinchunggan-tang is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis. |
| Key words:
Injinchunggan-tang(Yinchenqinggan-tang), liver cirrhosis, liver fibrosis, Hepatic Stellate Cell(HSC), HSC-T6. |
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