Effect of <i>Lindera obtusiloba</i> extract on cancer metastasis

Yun, Hyuk; Lee, Yong-jae; Seo, Hyun-won; Park, Kyoung-jae; Ko, Ha-neul; Cha, Dong-seok; Kwon, Jin; Jeon, Hoon; Kim, Kang-san

The Journal of Internal Korean Medicine > Volume 33(4); 2012 > Article

Original Article

The Journal of Internal Korean Medicine 2012;33(4): 405-417.

Effect of Lindera obtusiloba extract on cancer metastasis

Hyuk Yun¹, Yong-jae Lee³, Hyun-won Seo³, Kyoung-jae Park³, Ha-neul Ko¹, Dong-seok Cha³, Jin Kwon⁴, Hoon Jeon³, Kang-san Kim^1,2

¹Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University
²Research Center of Traditional Korean Medicine
³College of Pharmacy, Woo-Suk University
⁴Dept. of Prosthetics and Orthotics, Korea National College of Rehabilitation and Welfare

Correspondence

Kang-san Kim ,Tel: 063-859-2803, Email: bandit77@hanmail.net

Published online: December 30, 2012.

ABSTRACT

Objectives:
In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated.

Methods :
To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO.

Results:
1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of activation of NF-κB in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo.

Conclusions:
The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L. obtusiloba could be a valuable anti-metastatic agent.

Key words: Lindera obtusiloba, matrix metalloproteinase (MMP), cancer metastasis