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The Journal of Internal Korean Medicine 2012;33(4): 465-475. |
| 담관암세포주에서 地楡 에탄올 추출물의 항암 효과 |
| 박진규, 김영철 |
| 경희대학교 대학원 한의학과 간계내과학교실 |
| Pro-apoptotic Effects of Sanguisorbae Radix Ethanol-Extracts on Two
Cholangiocarcinoma Cell Lines, SNU-1079 and SNU-1196 |
| Jin-kyu Park, Young-chul Kim |
| Dept. of Internal Medicine, Oriental Medicine Graduate School, Kyung-Hee University |
| Correspondence |
Young-chul Kim ,Tel: 02-958-9118, Fax: 02-958-9120, Email: yckim@khmc.or.kr
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Published online: December 30, 2012. |
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| ABSTRACT |
Objectives:
This study was performed to investigate the antineoplastic effect of ethanol extracts from Sanguisorbae Radix on cholangiocarcinoma cells that was established from biliary tract cancer tissue.
Materials and Methods :
Two cholangiocarcinoma cell lines, SNU-1079 and SNU-1196, were studied. The mRNA expression of Caspase 3, 8, 9, Bcl-2, Bax, P53, and P21 was examined by RT-PCR. Cell viability was determined by MTT assay. The cell cycle was analyzed by flow cytometry and apoptosis by cell death detection ELISA kit.
Results:
Proliferation of SNU-1079 and SNU-1196 was inhibited by Sanguisorbae Radix treatment in a dose-dependent manner. All cells treated with Sanguisorbae Radix showed increased dose- and time-dependent apoptosis. The expression of caspase 3, 8, 9, p53, and p21 was increased in all cells after the treatment of Sanguisorbae Radix. The expression of Bcl-2 was decreased in SNU-1196 and Bax expression was increased in all cells after the treatment of Sanguisorbae Radix.
Conclusions:
These results suggest that Sanguisorbae Radix would be beneficial in the treatment of cholangiocarcinoma. |
| Key words:
Sanguisorbae Radix, cholangiocarcinoma, apoptosis |
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