Effects of <i>Auklandia Lappa</i> on Dextran Sulfate Sodium-Induced Murine Colitis

Kim, So-yeon; Park, Jae-woo; Ryu, Bong-ha

The Journal of Internal Korean Medicine > Volume 34(2); 2013 > Article

Original Article

The Journal of Internal Korean Medicine 2013;34(2): 134-146.

唐木香이 DSS(Dextran sulfate sodium)로 유발된 염증성 장질환 동물모델에 미치는 영향

김소연¹, 박재우², 류봉하²

¹부산대학교 한의학전문대학원 한의과학과
²경희대학교 한의과대학 비계내과 교실

Effects of Auklandia Lappa on Dextran Sulfate Sodium-Induced Murine Colitis

So-yeon Kim¹, Jae-woo Park², Bong-ha Ryu²

¹Dept. of Korean Medical Science, School of Korean Medicine, Pusan Naftional University
²3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University

Correspondence

Bong-ha Ryu ,Tel: 02-958-9140, Email: rbongh@khu.ac.kr

Published online: June 30, 2013.

ABSTRACT

Objectives:
Auklandia Lappa (ALE) is one of the herbs used frequently to treat abdominal pain and diarrhea and reported anti-inflammatory activity by suppressing proinflammatory cytokines. This study was designed to investigate whether ALE could show protective activities on experimental colitis induced by dextran sulfate sodium (DSS) models.

Methods :
Colitis was induced by DSS in Balb/c mice. ALE 10, 30, 100 and 300 mg/kg were orally administered twice a day for 7 days in DSS model. Mice weight was measured daily. Scoring of clinical findings was measured every other day. Colon length, edema, fecal blood and histological damages were assessed at day 7 in DSS model. In histological analysis, we checked cryptal glands, surface epithelium, submucosa, transmural, stroma and scored degree of inflammatory cell damage by modified histological scoring. We also calculated cytokines concentrations including IFN-γ, TNF-α, IL-6, IL-1β, IL-17, IL-23, IL-10 and TGF-β1 by Biometric Multiplex Cytokine Profiling method.

Results:
ALE showed the protective effects on DSS-induced experimental colitis. ALE inhibited shortening of colon length and histological damages of colon does-dependently, but it did not inhibit weight loss. ALE also inhibited IFN-γ and IL-6 expression, and upregulated cytokines (IL-10, TGF-β1) related to regulatory T cell differentiation and proliferation.

Conclusions:
The current results demonstrate the clinical utility of ALE in traditional medicine and indicate the possibility of potent drug development of inflammatory bowel diseases from natural products. Further investigations for exact mechanisms will be needed.

Key words: Auklandia Lappa, inflammatory bowel disease, dextran sulfate sodium, multiplex immunoassay