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The Journal of Internal Korean Medicine > Volume 35(3); 2014 > Article
The Journal of Internal Korean Medicine 2014;35(3): 262-273.
酒蒸黃連이 iNOS 활성 억제를 통해 생쥐 위⋅십이지장 점막에 미치는 영향
김명호, 임성우
동국대학교 한의과대학 내과학교실
Effect of Coptidis Rhizoma Steamed with Rice Wine on Gastroduodenal Mucosa of Mouse through Inhibiting iNOS Activation
Myung-ho Kim, Seong-woo Lim
Dept. of Internal Medicine, College of Oriental Medicine, Dong-Guk University
Correspondence  Seong-woo Lim ,Tel: 031-961-9046, Fax: 031-961-9045, Email: omdlsw@naver.com
  Published online: September 30, 2014.
ABSTRACT
Objectives:
This study was carried out to investigate the protective effect of Coptidis Rhizoma steamed with rice wine (CR) against gastroduodenal mucosal injury through inhibiting inducible nitric oxide synthase (iNOS) activation.

Methods:
In in vitro experiment, LPS-induced RAW 264.7 macrophages were treated with CR(0.4, 0.6, 0.8, 1.0 mg/ml) and iNOS mRNA expression and nitric oxide (NO) production were measured. In in vivo experiment normal group mice were treated with neither ethanol nor CR. Both control and sample group mice were orally administrated with ethanol. Five hours after ethanol administration control group mice were orally administrated with distilled water, sample group mice were orally administrated with CR. After three days administration, gastroduodenal mucosa of mice was observed histopathologically and iNOS, nuclear factor-kappa B (NF-κB) activation were observed immunohistochemically.

Results:
In in vitro experiment iNOS mRNA expression and NO production in LPS-induced RAW 264.7 macrophages were decreased by CR dose-dependently. In in vivo experiment, gastroduodenal mucosal injury was repaired by CR and iNOS, NF-κB activation in gastroduodenal mucosa were decreased by CR.

Conclusions:
Coptidis Rhizoma steamed with rice wine has a protective effect against gastroduodenal mucosal injury through inhibiting iNOS activation.
Key words: Coptidis Rhizoma steamed with rice wine, Gastroduodenal Ulcer, Nitric Oxide, Inducible Nitric Oxide Synthase, Nuclear Factor kappa B
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