Study on Anti-Cancer Effects of <i>Rhus Verniciflua</i> Stokes Extracted with Sterile Distilled
Water on Two Cholangiocarcinoma Cell Lines, SNU-1079 and SNU-1196

Joung, Bo-bae; Kim, Young-chul

The Journal of Internal Korean Medicine > Volume 36(1); 2015 > Article

Article

The Journal of Internal Korean Medicine 2015;36(1): 1-12.

漆皮 추출물의 담도암 세포주 SNU-1079와 SNU-1196에 대한 항암효과

정보배, 김영철

경희대학교 한의과대학 간계내과학교실

Study on Anti-Cancer Effects of Rhus Verniciflua Stokes Extracted with Sterile Distilled Water on Two Cholangiocarcinoma Cell Lines, SNU-1079 and SNU-1196

Bo-bae Joung, Young-chul Kim

Dept. of Internal Medicine, College of Korean Medicine, Kyung-Hee University

Correspondence

Young-chul Kim ,Tel: 02-958-9118, Fax: 02-958-9120, Email: yckim@khmc.or.kr

Received: January 12, 2015, Accepted: March 17, 2015, Published online: March 30, 2015.

ABSTRACT

Objectives:
This study was performed to investigate the anti-cancer effects of Rhus verniciflua Stokes (RVS) extracted with sterile distilled water on cholangiocarcinoma cell lines. Materials and

Methods:
Two cholangiocarcinoma cell lines, SNU-1079 and SNU-1196, were used in this study. Cells were treated with different concentrations of RVS for 24, 48, and 72 hours. Cell count, viability, apoptosis, and mRNA expression of Bax, Bcl-2, Mcl-1, survivin, caspase-3, and cyclin D1 and P21 were determined with an automatic cell counter (ADAM-MC), MTT assay, apoptosis assay (Annexin-V/PI staining), and RT-PCR.

Results:
All cells treated with RVS showed decreased cell counts in a dose-dependent manner. RVS inhibited proliferation of SNU-1196 in a dose-dependent manner, but SNU-1079 proliferation was inhibited in the long-time culture group in a dose-dependent manner. The proportion of early and late-stage apoptotic cells was increased by RVS in a dose-dependent manner in SNU-1196. In contrast, it was increased significantly in SNU-1079 treated with high-dose RVS. After treatment with RVS, the mRNA expression of Bcl-2 was decreased while Bax was increased in SNU-1079. Cyclin D1 mRNA levels were decreased in SNU-1196 in a dose-dependent manner. P21 expression was increased in all cells after the treatment with RVS.

Conclusions:
RVS appears to have potential as a therapeutic agent for cholangiocarcinoma.

Key words: Rhus verniciflua Stokes, Cholangiocarcinoma, Apoptosis