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The Journal of Internal Korean Medicine 2015;36(2): 189-199. |
| 산양산삼복합약침의 표준화 및 급성독성시험 |
| 백상현1, 이인희2, 김민정2, 김은지2, 하인혁2, 이진호2, 이재웅2 |
1강남자생한방병원 한방내과
2자생한방병원 R&D센터 |
| Component Analysis and Toxicity Study of Combined Cultivated Wild Ginseng
Pharmacopuncture |
| Sang-hyun Baek1, In-hee Lee2, Min-jeong Kim2, Eun-jee Kim2, In-hyuk Ha2, Jin-ho Lee2, Jae-woong Lee2 |
1Dept of Internal Medicine, Jaseng Hospital of Korean Medicine
2Jaseng Spine and joint Research Institute, Jaseng Medical Foundation, Jaseng Hospital of Korean Medicine |
| Correspondence |
Jae-woong Lee ,Tel: +82-2-3218-2153, Fax: 02-3445-6644, Email: leejw@jaseng.co.kr
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Received: June 5, 2015, Accepted: June 25, 2015, Published online: June 30, 2015. |
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| ABSTRACT |
Objectives:
The marker substances of cultivated wild ginseng pharmacopuncture that may not be detected during the process of steaming remain controversial. We developed a combined cultivated wild ginseng pharmacopuncture that contains all the marker substances. The aim of this experiment was to investigate the marker substances and test the toxicity of the combined cultivated wild ginseng pharmacopuncture.
Methods:
The marker substances were detected using HPLC. Intravenous injection toxicity studies were conducted at Medvill, an authorized institution for non-clinical studies, under the regulations of Good Laboratory Practice. We observed survival rates, abnormal behaviors, weight changes, gross findings in autopsy, blood biochemical properties, and histological abnormalities of organs such as the liver and kidney.
Results:
HPLC data showed that ginsenosides Rg1, Rb1, and Rg3 were detected at concentrations of 19.29, 47.64, and 3.02 μg /ml, respectively. Administration of combined cultivated wild ginseng pharmacopuncture resulted in no dead animals or significant toxicological changes.
Conclusions:
The combined cultivated wild ginseng pharmacopuncture contains all the marker substances and is a relatively safe treatment medium. Further studies should be conducted to confirm the present findings. |
| Key words:
combined cultivated wild ginseng pharmacopuncture, component analysis, toxicity study |
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