| Hep G2 세포에서 간염제1탕의 에탄올에 의한 세포독성 억제효과 |
| 나기웅, 박용권, 김강산, 강병기 |
| 원광대학교 한의과대학 간계내과학교실 |
| The Effect of Hepatitis Treatment-Tang No.1 on Ethanol-Induced Cytotoxicity of Hep G2 Cells |
| Ki-Ung Ra, Young-Kweon Park, Gang-San Kim, Byung-Ki Kang |
| Department of Internal Medicine, College of Oriental Medicine, Wonkwang University |
| Correspondence |
Ki-Ung Ra ,
|
|
|
Received: May 12, 2001, Accepted: June 15, 2001, Published online: March 30, 2001. |
|
|
|
| ABSTRACT |
Object:
Hepatitis Treatment-tang No.1 has been used for the treatment of Liver disease and Jaundice. Long-term EtOH exposure leads to immunoregulatory and detoxification impairment. This study aimed to determine the relationship between TNF-α production and expression, and EtOH-induced cytotoxicity on Hep G2 cells.
Method:
Cells were incubated with EtOH in the presence or absence of HT. The cells were tested after 24 hours and, again, after 48 hours. Cytoviability and TNF-α release were analyzed by MTT assay and enzyme linked immunosorbent assay (ELISA), respectively. After 24 hours of EtOH exposure, the cytoviability decreased, and the release of TNF-α was increased. Increased amounts of TNF-α contribute to EtOH-induced cytotoxicity. The Anti-TNF-α antibody almost abolished it. Interestingly, EtOH-induced cytotoxicity and TNF-α production were inhibited by HT. Moreover, when HT was used in combination with the anti-TNF-α antibody, there was a marked inhibition of EtOH-induced cytotoxicity.
Results:
These results suggest that HT may prevent the cytotoxicity through partial inhibition of the TNF-α secretion. |
| Key words:
Hepatitis Treatment-tang No.1, Cytotoxicity, TNF-α, Hep G2 Cells. |
|
PDF Links
Full text via DOI 
Download Citation 
