| 고삼이 H2O2에 인한 대동맥평활근 세포의 고사에 미치는 영향 |
| 차용석1, 정승원1, 허윤1, 이대용1, 송영준2, 나헌식2, 이주헌2, 문병순1 |
1원광대학교 한의학전문대학원
2원광대학교 한의과대학 심계내과학교실 |
| Effects of Sophorae Radix on H2O2-induced Apoptosis in Aortic Vascular Smooth Muscle Cells |
| Yong-Seok Cha1, Seung-Won Jeong1, Yoon Hoe1, Dae-Yong Lee1, Young-Jun Song2, Heon-Sik Na2, Joo-Heon Lee2, Byung-Soon Moon1 |
1Professional Graduate School of Oriental Medicine, Wonkwang University
2Department of Internal Medicine, College of Oriental Medicine, Wonkwang University |
|
|
|
Received: September 3, 2002, Published online: December 30, 2002. |
|
|
|
| ABSTRACT |
Objective :
Apoptosis of vascular smooth muscle cells(VSMCs) is essential in atherogenesis, as a factor that modulates its early progression rather than a terminal event in the course of the disease. Various stimuli, including oxide lipoproteins, altered hemodynamic stress and free radical, induced VSMCs apoptosis in vitro. The protective effects of Sophorae Radix(SR) on apoptotic cell death induced by H2O2 were investigated in VSMCs.
Method:
The cultured cells were pre-treated with SR and exposed to H2O2. The cell damage was assessed by using MTT assay. Also, we used Hoechst stain, Western blotting analysis. .
Result:
SR protected the H2O2-induced apoptotic death of VSMCs, which was characterized as ladder-pattern fragmentation of genomic DNA. SR decreased the activation of caspase-3 like protease induced by H2O2 and recovered control level from H2O2-induced Bax and Fas expression and mitochondrial membrane potential.
Conclusion:
SR protected VSMCs apoptotic death induced by H2O2 via inactivation of caspase-3, modulation of mitochondrial function and regulation of Fas expression. |
| Key words:
Sophorae Radix, H2O2-induced apoptosis, vascular smooth muscle cells |
|
PDF Links
Full text via DOI 
Download Citation 
