| 소속명탕이 저산소증으로 유발된 대뇌신경세포의 손상에 미치는 영향 |
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| Effect of Sosokmyeung-tang on the Cultured Cerebral Neurons Damaged by Hypoxia |
| Chang yong Lim, Kwang Ro Lee, Sang Su Cheong, Sei Young Kang, So Young Lee, Dae Yong Lee, Sang Kwan Lee, Kang Keyng Sung |
| Department of Neuroscience and Internal Medicine, College of Orinental Medicine, Wonkwang University |
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Published online: September 30, 2003. |
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| ABSTRACT |
Objectives:
This study was performed to elucidate the mechanism of hypoxia on cultured cerebral neurons of mouse.
Method:
The cytotoxic effect of hypoxia was measured in these cultures by succinic dehydrogenase activity(SDA), lysosomal enzyme activity(LEA), neurofilament(NF) enzymeimmuno assay(EIA), catalase(CAT) activity, protein syntheses, LDH activity and protein kinase C(PKC) activity after cerebral neurons were respectively exposed to hypoxia for from one to sixty minutes. In addition, the protective effect of Sosokmyeung-tang(SST) extracts on hypoxia-induced neurotoxicity was examined in these cultures.
Result:
1. Hypoxia induced the decrement of SDA and LEA by colorimetric assay after cultured cerebral neurons were respectively exposed to hypoxic condition from five to twenty minutes, respectively in these cultures. 2. Hypoxia-induced neurotoxicity decreased the protein synthesis, CAT activity and amount of neurofilament, but increased the LDH activity and PKC activities in these cultures. 3. In effect of SST extracts on hypoxia-induced neurotoxicity, it showed positive effect in blocking hypoxia-induced cytotoxicity by the increase of amount of neurofilament, and protein synthesis and CAT activity, and by the decrease of LDH and PKC activities in these cultures.
Conclusions:
It suggests that brain hypoxia has neurotoxic effect on cultured mouse cerebral neurons, and the extracts of SST was very effective in the protection of hypoxia-induced neurotoxicity in these cultures. |
| Key words:
Sosokmyeung-tang, Hypoxia, cytotoxic effect |
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