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The Journal of Internal Korean Medicine 2004;25(2): 214-223. |
| 斑蝥가 대장암세포에 미치는 영향 |
| 김현일, 김진성, 윤상협, 류봉하, 류기원 |
| 경희대학교 의과대학 의료원 3내과 |
| Effect of Mylabris phalerata on colorectaladenocarcinoma cells (SNU-C5 cell lines) |
| Hyeon-Yil Kim, Jin-Sung Kim, Sang-Hyub Yoon, Bong-Ha Ryu, Ki-Won Ryu |
| Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University |
| Correspondence |
Hyeon-Yil Kim ,Tel: 02-958-9140, Fax: 02-958-9136, Email: khymkm@hanmir.com
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Received: April 10, 2004, Accepted: May 6, 2004, Published online: June 30, 2004. |
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| ABSTRACT |
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The study examines the anti-cancer effects of the hot water extract of Mylabris phalerata(MP) using SNU-C5 cell lines. Microscopic analysis showed that 12 hours after MP treatment, the number of dead cells increased prominently. Significant cell death was observed 12, 24, and 48 hours after MP treatment through trypan blue exclusion testing. This suggests that MP is time-dependently cytotoxic. Mitotracker Red CMXRos staining and flowcytometry revealed that MP decreased mitochondrial membrane potentials. The absence of peaks on PI staining showed that DNA damage occurred in MP treated cells. Taken together, measurements suggest that MP has a strong anti-cancer effect on SNU-5 cell lines, and that this is likely to be due to the destruction of mitochondria and DNA damage. |
| Key words:
My labris phalerata, membrane potentials. |
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