Radioprotective Effect of Saengmaek-san on Mice Jejunal Crypt Cell Survival and Apoptosis

Kim, Hyun-Kyung; Kim, Jin-Sung; Yoon, Sang-Hyub; Ryu, Bong-Ha

The Journal of Internal Korean Medicine > Volume 27(2); 2006 > Article

Original Article

The Journal of Internal Korean Medicine 2006;27(2): 316-326.

生脈散의 방사선 보호효과 : 생쥐 소낭세포 재생과 Apoptosis에 미치는 영향

김현경, 김진성, 윤상협, 류봉하

경희대학교 한의과대학 비계내과학 교실

Radioprotective Effect of Saengmaek-san on Mice Jejunal Crypt Cell Survival and Apoptosis

Hyun-Kyung Kim, Jin-Sung Kim, Sang-Hyub Yoon, Bong-Ha Ryu

3rd Deptartment of Internal Medicine, College of Oriental Medicine, Kyunghee University

Correspondence

Jin-Sung Kim ,Tel: 02-958-8895, Fax: 02-958-9136, Email: oridoc@chollian.net

Received: April 15, 2006, Accepted: June 13, 2006, Published online: June 30, 2006.

ABSTRACT

Backgrounds & Objects:
The aim of this study was to investigate the radioprotective effect of Shengmai-san(SMS), a herbal medicine, on mice jejunal crypt cell survival and Apoptosis.

Methods:
Mice were devided into 4 groups according to radiation dose and SMS treatment; Normal was the group without irradiation. Control was the group treated with D.W before 10 Gy irradiation. SMS 2.9 was sample group treated with 2.9 mg/10 g of SMS extract before 10 Gy irradiation and SMS 29 was sample group treated with 29 mg/10 g of SMS extract before 10 Gy irradiation. And Each group were sacrificedat 24 hours and 72 hours after irradiation. To analyze the crypt survival, hematoxylin-eosin staining was used and to analyze the apoptosis, the TUNEL assay was done.

Results:
1. From the microcolony survival assay, the SMS 2.9 and SMS 29 showed the radioprotective effect with a statistical significance compared to the control group at 24 hr (p < 0.01) and 72 hr (p < 0.001) after 10 Gy irradiation. And the differences of radioprotective effect between SMS 2.9 and SMS 29 were not significant. 2. The results of the TUNEL assay showed that the apoptotic index in SMS 2.9 and SMS 29 was significantly decreased, as compared to the control group at both 24 hr ( p < 0.01) and 72 hr (SMS 2.9 : p < 0.001, SMS 29 : p < 0.01) after 10Gy irradiation. And the differences of between SMS 2.9 and SMS 29 were not significant.

Conclusions:
It could be suggested that the Shengmai-san has a prominent protective effect in mice intestines against the radiation damage. And the radioprotective effect seems to be related to inhibition of the apoptosis.

Key words: Saengmaek-san, radioprotective effect, apoptosis