| 生肝健脾湯이 HepG2 cell의 증식, 세포사멸 및 활성조절 신호전달계에 미치는 영향 |
| 김재용, 김영철, 이장훈, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| The Effects of Saengkankunbi-tang on Proliferation, Apoptosis and Cell Signaling Pathways of HepG2 Cells |
| Jae-yong Kim, Young-chul Kim, Jang-hoon Lee, Hong-jung Woo |
| Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University |
| Correspondence |
Hong-jung Woo ,Tel: 02-958-9118, Fax: 02-958-9120, Email: hjwoo@khu.ac.kr
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Received: February 10, 2006, Accepted: February 27, 2006, Published online: March 30, 2006. |
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| ABSTRACT |
Objectives:
This study was done to evaluate the effects of Saengkankunbi-tang on cell-viability, proliferation, cell-cycle, apoptosis and DNA replication on HepG2 cell and to find out by which molecular-biological mechamism by which Saengkankunbi-tang operates.
Methods :
The MTT assay, cell counting assay, [3H]-thymidine incorporation assay, flow cytometric analysis, tryphan blue exclusion assay, western blot analysis, quantative RT-PCR were taken.
Results:
Saengkankunbi-tang had no effect on proliferation, cell-cycle and DNA replications of HepG2 cells, while it improved cell viability and reduced apoptosis, and it activated Akt and NFκB. But, it did not produce an effect on cell viability and apoptosis when PI3K/Akt pathway was blocked by LY294002 nor when NFκB activation was blocked by DN-IκB.
Conclusion:
These results suggests that Saengkankunbi-tang improves cell viability and reduces apoptosis of HepG2 cells, by activating NFκB through PI3K/Akt pathway. |
| Key words:
Saengkankunbi-tang, PI3K/Akt pathway, NFκB, apoptosis, HepG2 cell |
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