| 인체폐암세포 NCI-H460 및 A549의 apoptosis 유발에 미치는 參芪補肺湯의 영향 |
| 허만규, 허태율, 김기탁, 변미권, 김진영, 심성흠, 김광록, 감철우, 박동일 |
| 동의대학교 부속한방병원 폐계내과학 교실 |
| Induction of Apoptosis by Samgibopae-tang in Human Non-small-cell Lung Cancer Cells |
| Man-kyu Heo, Tae-yool Heo, Ki-tak Kim, Mi-kwon Byun, Jin-young Kim, Sung-heum Sim, Koang-lock Kim, Cheol-woo Kam, Dong-il Park |
| Department of Respiratory Internal Medicine, College of Oriental Medicine, Dong-Eui University |
| Correspondence |
Dong-il Park ,Tel: 051-850-8650, Fax: 051-867-5162, Email: dipark@deu.ac.kr
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Published online: September 30, 2007. |
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| ABSTRACT |
Objectives:
This study was designed to investigate the antiproliferative activity of the water extract of Samgibopae-tang (SGBPT) in NCI-H460 and A549 non-small-cell lung cancer cell lines.
Methods :
In this study, we measured the subsistence, form of NCI-H460 and A549 non-small-cell lung cancer cell by hemocytometer and DAPI staining. In each cell, we analyzed DNA fragmentation, reverse transcription-polymerase chain reaction and measured activity of caspase-3, caspase-8 and caspase-9.
Results and Conclusions :
We found that exposure of A549 cells to SGBPT resulted in growth inhibition in a dose-dependent manner, butSGBPT did not affect the growth of NCI-H460 cells. The antiproliferative effect by SGBPT treatment in A549 cells was associated with morphological changes. SGBPT treatment partially induced the expression of DR5 cells and the expression of Faswas markedly increased in both transcriptional and translational levels in A549 cells. SGBPT treatment partially induced the expression of Bcl-2, Bcl-XL and the expression of Bid was markedly decreased in translational levels in A549 cells. However, SGBPT treatment did not affect the expression of IAP family in A549 orNCI-H460 cells. SGBPT treatment partially induced the expression of caspase-3, caspase-8, caspase-9 activity which markedly increased in a dose-dependent manners in A549 cells. The fragmental development of PARP and β-catenin protein was observed in A549 cells by SGBPT treatment. SGBPT treatment induced the expression of PLC-γ1 protein which decreased in A549 cells. SGBPT treatment partially induced the expression of DFF45/ICAD which markedly increased in a dose-dependent manner in A549 cells. Taken together, these findings suggested that SGBPT-induced inhibition of human lung carcinoma did not affect NCI-H460 cell growth. However, SGBPT-induced inhibition of human lung carcinoma A549 cell growth was associated with the induction of death receptor and mitochondrial pathway. The results provided important new insights into the possible molecular mechanisms of the anti-cancer activity of SGBPT. |
| Key words:
Samgibopae-tang, non-small-cell lung cancer cells, NCI-H460, A549, apoptosis |
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