| 3-NP에 의해 유발된 신경교세포의 산화적 손상에 대한 南星의 보호효과 |
| 남상규1, 이정섭1, 신용진1, 전지영1, 설재균1, 최철원2, 신선호1, 이인1 |
1원광대학교 한의과대학 심계내과학 교실
2성심한의원 |
| The Neuroprotective Effect of Rhizoma Arisaematis on 3-NP-induced Oxidative Injury
of C6 Glial Cells |
| Sang-kyu Nam1, Jung-sup Lee1, Yong-jin Shin1, Ji-young Jeon1, Jae-gyun Seol1, Chul-won Choi2, Sun-ho Shin1, In Lee1 |
1Department of Internal Medicine, College of Oriental Medicine, WonKwang University
2SungShim Oriental Medical Clinic |
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Published online: September 30, 2007. |
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| ABSTRACT |
Objectives:
This study aimed to investigate the underlying protective mechanism of Rhizoma Arisaematis(RA) on 3-NP-induced cytotoxicity in rat C6 glial cells.
Methods :
We investigated treatment ofC6 cells with 20mM 3-NP and pretreatment with RA to cause loss of cell viability, and morphological change, which was associated with elevation of ROS level, increase in Bax/Bcl2 ratio and HIF-a protein expression.
Results:
RA inhibited 3-NP-induced cell death in C6 glial cells and inhibited the changes of the MMPT (mitochondria membrane potential transition) and inhibited the decrease of mitochondria complex II activity and 3-NP-induced ROS generation in C6 cells. And RA decreased the activity of HIF-a and Bax, and increased the activity of Bcl2 in C6 glial cells.
Conclusions:
RA markedly protects C6 glial cells from 3-NP-induced oxidative injury. |
| Key words:
Rhizoma Arisaematis, oxidative stress, 3-NP |
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