| Splenocyte, BMMC, eosinophil을 통해 본 丹蔘이 천식 관련 염증세포에 미치는 영향 |
| 정승연1, 김진주2, 정희재1, 정승기1 |
1경희대학교 한의과대학 폐계내과학 교실
2경희대학교 약학대학 한방생리학교실 |
| Effects of Salviae miltiorrhizae on Inflammatory Cells Associated with Asthma via
Splenocyte, BMMC, Eosinophil |
| Seung-yeon Jeong1, Jin-ju Kim2, Hee-jae Jung1, Sung-ki Jung1 |
1Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University
2Dept. of Oriental Physiology, College of Pharmacy, Kyung-hee University |
| Correspondence |
Sung-ki Jung ,Tel: 02-958-9147, Fax: 02-958-9148, Email: jskes@unitel.co.kr
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Published online: March 30, 2009. |
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| ABSTRACT |
Objectives:
Asthma is a chronic inflammatory disorder of the airways by many cells such as mast cells, Th2 lymphocytes and eosinophils. The present study was aimed to evaluate the effects of Salviae miltiorrhizae (SM) on T cell cytokine production, mast cells, and eosinophils.
Methods :
We screened 13 herbs to find compounds with potential to control Th cytokine production, using concanavalin A (con A)-activated splenocyte cultures. Con A-activated IFN-γ and IL-4 levels in supernatants of splenocyte cultures. Bone marrow derived mast cells (BMMC) were incubated with SM and then the expressions of membrane proteins of BMMC were analyzed by fluorescence activated cell sorter (FACS). BALB/c mice sensitized to ovalbumin (OVA) were challenged with aerosolized OVA for 6 weeks. During the last weeks some mice were treated with SM. Then eosinophils in bronchoalveolar lavage fluid (BALf) were counted and pathologic changes of lung tissue were observed with hematoxylin-eosin stain.
Results:
SM increased IFN-γ level on splenocyte culture significantly, but had no significant effects on expressions of ICAM-1, CD62L, integrin α4, c-kit, IL-3 receptors, CD11a, or IgE receptors of BMMC. SM treatment significantly inhibited eosinophil infiltrates in BALf and peribronchial lung inflammation.
Conclusions:
The present data suggested that SM may have an effect on Th cytokine secretion and eosinophils associated with asthma responses. Therefore SM might be of therapeutic value in treating asthma. |
| Key words:
asthma, Salviae miltiorrhizae, Th cytokine, mast cell, eosinophil |
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