우황청심환이 저산소증 유발 배양신경세포에 미치는 영향 |
조규선1, 정승현1, 신길조1, 문일수2, 이원철1 |
1동국대학교 한의과대학 내과학교실 2동국대학교 의과대학 해부학교실 |
The Effects of Woohwangcheongsim-won on Hypoxic E18 Cortical Neuroblast |
Gyu-Seon Cho1, Sung-Hyun Chung1, Gil-Cho Shin1, Il-Soo Moon2, Won-Chul Lee1 |
1Department of Internal Medicine, College of Oriental Medicine, Dongguk University 2Department of Anatomy, College of Medicine, Dongguk University |
Correspondence |
Sung-Hyun Chung ,
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Received: June 30, 2001, Accepted: July 21, 2001, Published online: June 30, 2001. |
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ABSTRACT |
Objectives: The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast.
Methods : To evaluate the effect of Woohwangcheongsim-won on neuronal death caused by hypoxia, the survival rate of E18 cortical neuroblast was measured with MTT assay and the changes of several synaptic proteins and enzymes were investigated with the immunoblot assays.
Results: The E18 cortical neuroblasts were added 50, 100, 500, 1,000, and 5,000㎍/㎖ Woohwangcheongsim-won. They showed neurotoxicity, when the concentration of Woohwangcheongsim-won was above 1,000㎍/㎖ . The E18 cortical neuroblasts, which were added 50, 100, and 500㎍/㎖ Woohwangcheongsim-won, were exposed 98% N2/5% CO2 for 3 hours to induce hypoxia. 3 days later, the survival rate of 50㎍/㎖ Woohwangcheongsim-won was 141.5% when compared to the control group. On the immuneblot assays, the expressions of αCaMKII, NR2A, NR2B, PDE2, PSD-95, and eEF-1α were increased in normoxia, but those of NR2A, NR2B were decreased in hypoxia when compared to the control group.
Conclusions: The data shows that the effects of Woohwangcheongsim-won on neuronal death of hypoxic E18 cortical neuroblast is a significant result. |
Key words:
Woohwangcheongsim-won, hypoxic cortical neuroblast, immunoblot |
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