The Molecular Biological Study on Anti-Cancer Effects of <i>Sagunjatang plus Cremastrae Appenediculatae Tuber</i> on Human Stomach Cancer Cells

Kim, Jin-Seok; Ryu, Bong-Ha; Ryu, Ki-Won; Yoon, Sang-Hyub; Kim, Jin-Seong

The Journal of Internal Korean Medicine > Volume 23(2); 2002 > Article

Original Article

The Journal of Internal Korean Medicine 2002;23(2): 202-211.

사군자탕가산호고가 위암세포에 미치는 항암효과에 대한 분자생물학적 연구

김진석, 류봉하, 류기원, 윤상협, 김진성

경희대학교 한의과대학 간계내과학교실

The Molecular Biological Study on Anti-Cancer Effects of Sagunjatang plus Cremastrae Appenediculatae Tuber on Human Stomach Cancer Cells

Jin-Seok Kim, Bong-Ha Ryu, Ki-Won Ryu, Sang-Hyub Yoon, Jin-Seong Kim

Dept. of 3rd Internal Medicine, College of Oriental Medicine, Kyunghee University

Correspondence

Jin-Seok Kim ,Tel: 02-958-9140, Fax: 02-958-9136, Email: yalliyalli70@hanmail.net

Received: April 9, 2002, Accepted: July 31, 2002, Published online: June 30, 2002.

ABSTRACT

Background:
The previous studies on anticancer medicine derived from korean traditional medicine have focused on the life elongation of cancer cell bearing animals. However, it is thought that more molecular biological studies are needed to reveal their mechanism. Objective:
The aim of this study was to investigate the molecular biological function of Sagunjatang plus Cremastrae Appenediculatae Tuber on cytostaticity, apoptosis and apoptosis related genes revelation against human stomach cancer cells(AGS).

Methods:
After administrating Sagunjatang and Sagunjatang plus Cremastrae Appenediculatae Tuber to human stomach cancer cell, MTT assay was performed to compare and examine the efficacy of each medicine on the cytostaticity of stomach cancer cells in proportion to time and doses, and apoptosis assay was performed to examine their effect on apoptosis by using DAPI dye and counting the number of cells which developed in an apoptotic body. In addition, the quantitative RT-PCR was used to examine their effect on the revelation of Bcl-2, Bax and P53, which are genes related to apoptosis.

Result and Conclusion:
Sagunjatang plus Cremastrae Appenediculatae Tuber demonstrated increased cytostaticity, decreased apoptosis and unremarkable revelation of apoptosis related genes. But in the cytostaticity and apoptosis, Sagunjatang plus Cremastrae Appenediculatae Tuber showed a tendency to control stomach cancer cells. Therefore, we can expect the clinical application to the related diseases. Besides, it needs another experiment on various cancer cells, such as, lung cancer cell and hysterocarcinoma cell.

Key words: Sagunjatang, Cremastrae Appenediculatae Tuber, Human stomach cancer cells(AGS), MTT assay, Apoptosis, RT-PCR