Effects of <i>Hyeolbuchukeo-Tang</i> on LDL oxidation in Macrophage Cell

Choi, You-Kyung; Go, Jae-Chul; Baek, Eun-Ki; Hong, Ui-Sil; Han, Ji-Wan; Park, Se-Ki; Kim, Dong-Woo; Jun, Chan-Yong; Han, Yang-Hee; Park, Chong-Hyeong

The Journal of Internal Korean Medicine > Volume 23(3); 2002 > Article

Original Article

The Journal of Internal Korean Medicine 2002;23(3): 406-414.

혈부축어탕이 대식세포의 oxLDL 생성능에 미치는 영향

최유경¹, 고재철², 백은기¹, 홍의실¹, 한지완¹, 박세기¹, 김동우¹, 전찬용², 한양희², 박종형¹

¹경원대학교 한의과대학 내과학교실
²경원대학교 인천한방병원 내과

Effects of Hyeolbuchukeo-Tang on LDL oxidation in Macrophage Cell

You-Kyung Choi¹, Jae-Chul Go², Eun-Ki Baek¹, Ui-Sil Hong¹, Ji-Wan Han¹, Se-Ki Park¹, Dong-Woo Kim¹, Chan-Yong Jun², Yang-Hee Han², Chong-Hyeong Park¹

¹Department of Internal Medicine, College of Oriental Medicine, Kyung-Won University
²Kyung-Won University Inchon Medical Center

Correspondence

You-Kyung Choi ,Tel: 02-425-3456, Fax: 02-425-3560, Email: pancoss@freechal.com

Received: July 11, 2002, Accepted: August 31, 2002, Published online: September 30, 2002.

ABSTRACT

Objectives:
With the increasing morbidity of cardiovascular diseases, both preventive and therapeutic attention is paid to arteriosclerosis. Many studies reveal that oxLDL plays an important role in accelerating arteriosclerosis. Clinically, to remove the coagulated blood and to facilitate blood circulation, Hyeolbuchukeo-Tang(HC) is prevalently prescribed. This study is aimed to observe the macrophage s suppressive activity of oxLDL formation.

Methods :
The RAW 264.7 cells, cultivated murine macrophage, were respectively injected to both control group and 1 ㎍/㎖ , 10 ㎍/㎖ sample group of HC and cultivated. Afterward LDL was injected to both groups and cultivated for 90 hours. Then MDA, proteolytic activity, quantity of Nitrite were measured. Again, both groups of HC cultivated in the same way were exposed to CuSO4 for 20 hours to induce cell damage. Each group s cell suvival rate, LDL leakage level, and activity of damaged macrophage were measured and compared.

Results:
1. HC suppressed the oxLDL formation of macrophage. 2. HC increased the NO formation of macrophage. 3. HC increased the survival rate of damaged macrophage. 4. HC suppressed the macrophage s LDH releasing activity induced by oxLDL. 5. HC restored the macrophage s activity decreased by oxLDL.

Conclusion:
Above data suggest that Hyeolbuchukeo-Tang is effective against arteriosclerosis.

Key words: Arteriosclerosis, Hyeolbuchukeo-Tang, Macrophage, oxLDL