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The Journal of Internal Korean Medicine 2003;24(1): 123-133. |
| 淸肝解酒湯이 alcohol 대사관련 유전자 및 apoptosis에 미치는 영향 |
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| Effects of Chungganhaeju-tang on Gene Expression of Alcohol-metabolizing Enzymes and Alcohol-induced Apoptosis |
| Young-Tae Kim, Young-Chul Kim, Jang-Hoon Lee, Hong-Jung Woo |
| Dept. of Internal medicine I, College of Oriental Medicine, Kyung Hee University, Seoul, Korea |
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Received: January 17, 2003, Accepted: February 7, 2003, Published online: March 30, 2003. |
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| ABSTRACT |
Objectives:
This study was designed to investigate the effects of Chungganhaeju-tang on expression of alcoholmetabolizing enzymes, cell viability and alcohol-induced apoptosis.
Materials and Methods :
For this study, the human hepatoma cell line HepG2 was used. HepG2 cells were treated with ethanol-or acetaldehyde, chungganhaeju-tang, anti-Fas neutralizing antibody and were investigated by using quantitative RTPCR, MTT and Trypan blue exclusion assays.
Results:
The results are summarized as follows : 1. Quantitative RT-PCR analysis demonstrated that ethanol-or acetaldehyde-mediated increase of ALDH gene expression was not affected by Chungganhaeju-tang treatment. 2. Ethanol-or acetaldehyde-induced apoptosis was remarkably inhibited by Chungganhaeju-tang in a dose-dependent manner. 3. Ethanol- or acetaldehyde-induced apoptosis was significantly blocked by anti-FasL neutralizing antibody, suggesting apoptosis induced by alcohol might be mediated by FasL/Fas signaling pathway.
Conclusions:
Taken all together, these results indicate that the FasL/Fas signaling plays a critical role in alcohol-induced apoptosis and Chungganhaeju-tang increases viability of liver cells by suppression of the FasL/Fas-mediated apoptosissignaling pathway. |
| Key words:
Chungganhaeju-tang(Qingganjiejiu-tang), ALDH(aldehyde dehydrogenase), HepG2, Fas, apoptosis |
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