Effect of Yungmijihwang-tang and Bojungikki-tang on the Population Doublings and the Population Doubling Time in Rat Fibroblast, Heart-Endothelial Cells, Mesangial Cells

Seong, Ki-Won; Ahn, Young-Min; Ahn, Se-Young; Doo, Ho-Kyung

The Journal of Internal Korean Medicine > Volume 24(3); 2003 > Article

Original Article

The Journal of Internal Korean Medicine 2003;24(3): 551-560.

육미지황탕 및 보중익기탕이 Rat 의 피부섬유아세포, 사구체메산지움세포 및 혈관내피세포의 노환지연에 미치는 영향

Effect of Yungmijihwang-tang and Bojungikki-tang on the Population Doublings and the Population Doubling Time in Rat Fibroblast, Heart-Endothelial Cells, Mesangial Cells

Ki-Won Seong, Young-Min Ahn, Se-Young Ahn, Ho-Kyung Doo

Department of 6th Internal Medicine, College of Oriental Medicine, Kyung-Hee University, Seoul, Korea

Correspondence

Ki-Won Seong ,Tel: 032-435-2031, Email: aow@dreamwiz.com

Published online: September 30, 2003.

ABSTRACT

Objectives:
Yungmijihwang-tang and Bojungikki-tang are representative oriental prescriptions that promote the functions of Spleen and Kidney. They have been used in oriental medicine as drugs for producing and nourishing essence-and-blood that have key roles in delaying senility of human body. The purpose of this Study was to investigate effects of Yungmijihwangtang and Bojungikki-tang on delaying senility in rat fibroblast, heart-endothelial cells, mesangial cells.

Methods :
After feeding rats the drugs for 2 months, the fibroblast, heart-endothelial cells, mesangial cells were cultured and investigated. This experimental research is focused on measuring the Population Doublings(PDs) and the Population Doubling Time(PDT).

Results:
In vitro, Yungmijihwang-tang and Bojungikki-tang controlled the growth of fibroblast, heart-endothelial cells, mesangial cells, and extended the PDT of them. In fibroblasts, the PDs were increased and the PDT was decreased at passage- 1 in both Yungmijihwang-tang and Bojungikki-tang group, and passage-7 in Yungmijihwang-tang group(p<0.05). In heartendothelial cells, the PDs were increased and the PDT was decreased at passage-8 in Bojungikki-tang group(p<0.05). In mesangial cells, the PDs were increased and the PDT was decreased at passage-2 in both Yungmijihwang-tang and Bojungikki-tang group(p<0.05).

Conclusions:
According to the above results, it is shown that by both Yungmijihwang-tang and Bojungikki-tang, the PDs were increased and the PDT was decreased. It is concluded that both Yungmijihwang-tang and Bojungikki-tang have effects on delaying the secescence of rat fibroblast, heart-endothelial cells, mesangial cells.

Key words: Yungmijihwang-tang, Bojungikki-tang, Population Doublings(PDs), Population Doubling Time(PDT)