The Effects of <i>Saengkankunbi-tang</i> and Its Composition
on Free Fatty Acid-Induced Lipotoxicity in HepG2 Cell

Hong, Sung-in

The Journal of Internal Korean Medicine > Volume 34(1); 2013 > Article

Original Article

The Journal of Internal Korean Medicine 2013;34(1): 14-30.

HepG2 cell에서 유리지방산 유발 지방독성에 대한 生肝健脾湯과 일부 조성 한약물의 효과

홍성인

경희의료원 한방병원 동서협진실

The Effects of Saengkankunbi-tang and Its Composition on Free Fatty Acid-Induced Lipotoxicity in HepG2 Cell

Sung-in Hong

Dept. of East-West Integrated Medicine, Kyung-Hee University Medical Center

Correspondence

Sung-in Hong ,Tel: 02-958-1812, Fax: 02-958-9212, Email: sthong@khu.ac.kr

Published online: March 30, 2013.

ABSTRACT

Objectives:
The aim of this study was to investigate whether the effects of extract from Saengkankunbi-tang and its composition that Artimisiae capillaris Herba, Crataegi Fructus, Alismatis Rhizoma, Hoelen and Raphani Semen could protect HepG2 cells from palmitic acid-induced lipotoxicity through lysosomal and mitochondrial pathways in an in vitro model.

Methods :
To examine the effects of the extracts from Saengkankunbi-tang and its composition that Artimisiae capillaris Herba, Crataegi Fructus, Alismatis Rhizoma, Hoelen and Raphani Semen on palmitic acid-induced lipotoxicity in HepG2 cells, we measured the contents of cell viability, cytotoxicity. Then to investigate the effects of the extract from Saengkankunbi-tang, Artimisiae capillaris Herba and Raphani Semen, we measured that triglyceride, reactive oxygen species, ATP levels, glutathione levels, cytochrome c and cathepsin B.

Results:
The extracts from Saengkankunbi-tang and its composition had a cell-protective function. The extracts from Saengkankunbi-tang, Artimisiae capillaris Herba and Raphani Semen controlled triglyceride over-accumulation in cells and reduced overproduction of reactive oxygen species. The extracts from Saengkankunbi-tang and Raphani Semen increased ATP and glutathione levels which had been decreased by lipotoxicity. The extracts from Saengkankunbi-tang, Artimisiae capillaris Herba and Raphani Semen reduced leakage of cytochrome c and the extracts from Saengkankunbi-tang and Raphani Semen reduced leakage of cathepsin B in lipotoxicity.

Conclusions:
These results show that the extracts from Saengkankunbi-tang and its composition that Artimisiae capillaris Herba and Raphani Semen have cell protective effects on palmitic acid-induced lipotoxicity through lysosomal and mitochondrial pathways.

Key words: Saengkankunbi-tang, Artimisiae capillaris Herba, Raphani Semen, lipotoxicity