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The Journal of Internal Korean Medicine 2003;24(3): 426-440. |
| 석웅황의 시험관내 위암세포 항암작용에 관한연구 |
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| The Molecular Biological Study for Anti-Cancer effects of Realgar on AGS |
| Dong-Mook Kim, Jin-Sung Kim, Sang-Hyub Yoon, Bong-Ha Ryu, Ki-Won Ryu |
| Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung-Hee University |
| Correspondence |
Dong-Mook Kim ,Tel: 02-958-9140, Fax: 02-958-9136, Email: docmook@hanmir.com
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Published online: September 30, 2003. |
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| ABSTRACT |
Background & Objective :
Besides known effects of Korean traditional herbs, it was imperative to research for stronger anti-cancer drugs by conducting molecular biological studies. The Objective of this study was to find the effects of Realgar on AGS.
Materials & Methods :
AGS from American type was used as culture. Realgar caused morphological changes and the following tests were conducted. 1. Tryphan bule exclusin test : to measure the killing effect on AGS. 2. MTT Assay : to Measure suppressive effect. 3. Quantitative RT-PCR : Revelation of Bcl-2, Bcl-XL, Bax and Fas. 4. Mitochondrial membrance potential and permeability transition.
Results:
Realgar proved more positive effects than the controlled group. 1. Tryphan blue exclusin test : positive 2. MTT Assay : positive 3. Quantitative RT-PCR : decrease of Bcl-2 in proportion to concentration and increase of Fas. No change in Bax and Bcl- XL. 4. Realgar decreased the mitochondrial membrance potential.
Conclusion:
Basic Realgar experiment proved its significant effect on AGS. However, more researches are needed to practically identify its effects. |
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