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The Journal of Internal Korean Medicine 2002;23(4): 587-595. |
| 소암거담익기탕의 항암효과에 대한 연구 |
| 신병철, 윤상협, 류봉하, 류기원 |
| 경희대학교 한의과대학 비계내과학교실 |
| Experimental Studies on Anti-cancer Effects of Soamgudamikgitang in Mice |
| Byung-Chul Shin, Sang-Hyub Yoon, Bong-Ha Ryu, Ki-Won Ryu |
| Department of the 3rd Internal Medicine, College of Oriental Medicine, Kyung Hee University |
| Correspondence |
Byung-Chul Shin ,Tel: 02-958-9140, Fax: 02-958-9136, Email: sbc67@korea.com
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Received: September 3, 2002, Accepted: October 4, 2002, Published online: December 30, 2002. |
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| ABSTRACT |
Objective :
A series of experimental studies were conducted to prove the antitumor effectiveness of Soamgudamikgitang.
Methods :
Four-week-old C57BL/6 male mice were used in these studies. Experiments were conducted concerning the cytotoxic effect in vitro on B16-BL6 melanoma cells, the inhibitory effect in vitro on subfraction of DNA topoisomaerae I, the expression of metalloproteinase by RT-PCR method, the inhibiting effect in vitro on cell proliferation of BCE cells, the antiangiogenic effect in vitro in tube formation assay, the anti-angiogenic effect in vivo in chicken CAM assay, and the inhibitory effect in vivo on the metastasis of melanoma cell colonies.
Results:
Concerning the cytotoxic effect in vitro, the IC50 of Soamgudamikgitang on B16-BL6 melanoma cells was 1,500 / . Concerning the inhibitory effect on the subfraction of DNA topoisomerase I, the effect of Soamgudamikgitang proved to be lower than that of camptotecin. Concerning the expression of metalloproteinase by RT-PCR method, the group treated with Soamgudamikgitang showed a general decrease in the expression of MMP2. However, the same group was also inclined to show a relative increase in the expression of TIMP1. Concerning in vitro cell proliferation of BCE cells, Soamgudamikgitang showed statistically significant(p<0.05) concentration-dependent inhibition effect on cell proliferation. Concerning tube formation assay, Soamgudamikgitang showed a concentration-dependent inhibitory effect. In the chicken CAM assay, the group treated with 1mg of Soamgudamikgitang showed high angiotoxicity. However, the group treated with 100㎍ of Soamgudamikgitang showed an 80% inhibitory effect. Concerning in vivo experimental lung metastasis assay, the groups treated with Soamgudamikgitang respectively showed a inhibitory effect.
Conclusions:
The results of these experimental studies have proven the anti-metastatic effects of Soamgudamikgitang. Based on these results, our conclusion is that prescriptions containing Soamgudamikgitang will be highly effective in treating cancer patients. |
| Key words:
Soamgudamikgitang, Anti-cancer Effects |
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