The Effects of <i>Chungganhaeju-tang</i> (<i>Qingganjiejiu-tang</i>) on Alcoholic Liver Damages by Applying Proteomics

Jung, Yun-jong; Lee, Jang-hoon; Kim, Young-chul; Woo, Hong-jung

The Journal of Internal Korean Medicine > Volume 28(1); 2007 > Article

Original Article

The Journal of Internal Korean Medicine 2007;28(1): 68-78.

淸肝解酒湯이 알코올성 간손상 Proteome에 미치는 영향

정윤종, 이장훈, 김영철, 우홍정

경희대학교 한의과대학 간계내과학교실

The Effects of Chungganhaeju-tang (Qingganjiejiu-tang) on Alcoholic Liver Damages by Applying Proteomics

Yun-jong Jung, Jang-hoon Lee, Young-chul Kim, Hong-jung Woo

Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University

Correspondence

Jang-hoon Lee ,Tel: 02-958-9118, Fax: 02-958-9120, Email: komclive@khmc.or.kr

Published online: March 30, 2007.

ABSTRACT

Objectives:
The purpose of our study was to investigate the effects of Chungganhaeju-tang (Qingganjiejiu-tang) on alcoholic liver damage by applying proteomics.

Materials and Methods :
Sprague-Dawley rats were used in this experiment; the rats were divided into a control group, alcohol group and Chungganhaeju-tang + alcohol group. Ethanol was orally administered twice a day for 4 weeks to the alcohol group. Water without ethanol was administered twice a day for 4 weeks to the control group. Ethanol + Chungganhaeju-tang extract was orally administered twice a day for 4 weeks to the Chungganhaeju-tang + alcohol group. The livers of each group were processed and we investigated histology, OxyBlot, 2-dimensional electrophoresis, and western blot of liver of each group.

Results:
In the histological findings of the liver, the alcohol group showed portal fibrosis with a few septa or without septa. The Chungganhaeju-tang + alcohol group showed no fibrosis or portalfibrosis without septa. In the OxyBlot finding, Chungganhaeju-tangprevented liver damage by oxidation. In the 2-dimensional electrophoresis finding, formiminotransferase cyclodeaminase (FTCD), glucose regulated protein, 58 kDa (GRP58K), aryl sulfotransferase, sulfotransferase family 1A, member 2, similar to acyl-coenzyme A oxidase-like, and catalase were changed.

Conclusion:
Chungganhaeju-tangexerts an inhibitory effect against the fibrosis and oxidation induced by alcohol in rat liver cell, and some proteins induced by alcohol were changed by Chungganhaeju-tang.

Key words: Chungganhaeju-tang, proteomics, alcoholic liver damage